Quantitative Analysis of Ketoprofen Enantiomers by Liquid Chromatography/Electrospray Ionization-Mass Spectrometry

DOI

10.2991/bep-16.2017.41How to use a DOI?

Keywords

ketoprofen; stable isotope-coded derivatization; LC/ESI-MS; carboxylic acid enantiomers; saliva.

Abstract

A enantioselective and sensitive liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS) method is developed for the quantitative analysis of ketoprofen (KET) enantiomers based on a straightforward one-pot two-step stable isotope-coded derivatization of KET with a chiral derivatization reagent, (S)-1-methyl-4-(5-(3-aminopyrrolidin-1-yl)-2,4-dinitro- phenyl)piperazine, and methyl iodide, respectively. The processes introduce not only a chiral tagging to the analytes but also a quaternary amine to the analytes, making the analytes permanently charged. The OPTSICD enabled the highly sensitive detection and complete separation of the KET enantiomers. By using second derivatization step with methyl iodide-d3 (CD3I), a complete set of deuterated standards was utilized and used as internal standards. The use of deuterated KET-derivatives significantly improved the assay precision and accuracy. The developed method was successfully applied to the quantitative analysis of KET in human saliva.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Fen YANG
AU  - Dongri JIN
PY  - 2016/12
DA  - 2016/12
TI  - Quantitative Analysis of Ketoprofen Enantiomers by Liquid Chromatography/Electrospray Ionization-Mass Spectrometry
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 198
EP  - 201
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.41
DO  - 10.2991/bep-16.2017.41
ID  - YANG2016/12
ER  -