Electrochemical Study on A Heme-Histidine-Sodium Decyl Sulfate Nano Micelles Complex Artificial Peroxidase

DOI

10.2991/bep-16.2017.20How to use a DOI?

Keywords

heme; nano micelle; artificial peroxidase; electrochemistry

Abstract

A heme-histidine-sodium decyl sulfate nano micelles complex with peroxidase activity (MAP) was constructed self-assembly and immobilized on a hydroxy fullerene modified glassy carbon electrode. The cyclic voltammograms of the modified electrode with a pair of redox peaks were investigated. The formal potential of the MAP modified electrode was determined to be -0.312V, at a scan rate of 0.05V/s in 50 mmol/L sodium phosphate buffer solution (pH 8.0). The electron transfer rate constant (ks) and cathodic transfer coefficient (α) were 1.96s-1 and 0.4, respectively. The cathodic peak current of the MAP modified electrode responses linearly to hydrogen peroxide ranging from 300 µmol/L to 1700 µmol/L. The detection limit was concluded to be 32 µmol/L. The apparent Michaelis-Menten constant (Kmapp) could be determined to be 1.08 mmol/L.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Di LI
AU  - Yushuai ZHANG
AU  - Fangyong GENG
AU  - Baolin XIAO
AU  - Jun HONG
AU  - Tao HONG
PY  - 2016/12
DA  - 2016/12
TI  - Electrochemical Study on A Heme-Histidine-Sodium Decyl Sulfate Nano Micelles Complex Artificial Peroxidase
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 95
EP  - 99
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.20
DO  - 10.2991/bep-16.2017.20
ID  - LI2016/12
ER  -