Selection of Potential Probiotic Strains Isolated from Human Intestinal Tract and Traditional Ferment Milk

DOI

10.2991/bbe-16.2016.63How to use a DOI?

Keywords

Lactobacillus rhamnosus, Salmonella, Diarrhoea, Low pH, Bile salts.

Abstract

Diarrhea is the main reason of human deaths in developing countries, which also cause world-wide infant mortality. Diarrhoea occurs world-wide and causes 4% of all deaths and 5% of health loss to disability. It is most commonly caused by gastrointestinal infections which kill around 1.5 million people globally each year, mostly children in developing countries. In this study, five selected Lactobacillus isolated from human intestinal and ferment milk were preliminarily identified by 16S rDNA gene sequencing and assessed the ability to inhibit the infection of enteropathogens. The Lactobacillus strains were screened on the basis of probiotic characteristics (i.e., resistance to low pH and bile salts, adhesion to the human gastrointestinal tract, inhibition of pathogenic strains). Using an in vitro system simulating gastric transit, our findings indicated that the probiotic strains had the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells. It was demonstrated that the probiotic strains inhibited subsequent adhesion of pathogensto the HT-29 cell. Among the selected strains, the selected Lactobacillus rhamnosus F0533 showed a high probiotic potential and could be used in health-promoting food products.After analyzing the sequence of the 16SrDNA regions of these three strains, the potential probiotic F0533 were Lactobacillus rhamnosus.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Chaohui Xue
AU  - Chun Yue
AU  - Xueguo Liu
AU  - Le Yuan
PY  - 2016/07
DA  - 2016/07
TI  - Selection of Potential Probiotic Strains Isolated from Human Intestinal Tract and Traditional Ferment Milk
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 410
EP  - 416
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.63
DO  - 10.2991/bbe-16.2016.63
ID  - Xue2016/07
ER  -