Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

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Injury Mechanism of Sub-micron Calcium Oxalate Monohydrate and Dihydrate Crystals on Renal Epithelial Cells

Authors
Poonam Bhadja, Kai Yu, Xiuqun Yao, Jianming Ouyang
Corresponding Author
Poonam Bhadja
Available Online July 2016.
DOI
10.2991/bbe-16.2016.45How to use a DOI?
Keywords
Submicron Crystal, Concentration Effect, Cell Injury, Crystal Adhesion.
Abstract

Objective: This study aims to compare the cytotoxicity and adhesion of submicron calcium oxalate monohydrate (COM) and dehydrate (COD) crystals toward African green monkey renal epithelial cells (Vero) to reveal the mechanism of kidney stone formation at cellular level. Methods: The injury effect of COM and COD crystals in Vero was investigated by detecting the change of cell viability, lactate dehydrogenase (LDH) content and hyaluronan (HA) expression amount. The adhesion of crystals on cell surface was observed using scanning electron microscope; and the crystal adhesion amount was measured using inductively coupled plasma emission spectrometer. Results: The cellular injury induced by crystals was in a dose-dependent manner in the concentration range of 100~400 g/mL; COM caused more serious injury than COD, the adhesion amount of COM on Vero was significantly greater than COD. Conclusions: The damage of submicron COM is larger than submicron COD, the adhered COM crystals are more easily aggregated. The results in this paper indicated that the presence of high concentration of COM crystals in urine is more likely to increase the risk of stone formation than COD crystals.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
Series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
10.2991/bbe-16.2016.45How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Poonam Bhadja
AU  - Kai Yu
AU  - Xiuqun Yao
AU  - Jianming Ouyang
PY  - 2016/07
DA  - 2016/07
TI  - Injury Mechanism of Sub-micron Calcium Oxalate Monohydrate and Dihydrate Crystals on Renal Epithelial Cells
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 283
EP  - 289
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.45
DO  - 10.2991/bbe-16.2016.45
ID  - Bhadja2016/07
ER  -