Screening and Production Optimization of a New Organic Solvent-Tolerance Fungal Lipase

DOI

10.2991/bbe-16.2016.33How to use a DOI?

Keywords

Lipase, Screening, Organic solvents tolerance, Fermentation.

Abstract

A novel fungus which produced lipase extracellularly was isolated from soil. The strain was named after Candida tropicalis SD7 based on morphological features and ITS1-5.8S–ITS4 region sequences of ribosomal RNA. The fermentation conditions were optimized in order to improve lipase production of the strain. The maximum lipase activity was achieved at 72h. The optimum lipase production was observed in the medium with glycerol as carbon source and peptone as nitrogen source through submerged fermentation at 30°C and initial pH 7.0. The lipase showed stability in the presence of hydrophobic organic solvents such as methylbenzene, petroleum ether, isooctane and n-hexane. It was moderately stable in polar solvents, including DMSO, acetone, acetonitrile and methanol.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ren Peng
AU  - Binwen Hong
AU  - Pan Zhou
PY  - 2016/07
DA  - 2016/07
TI  - Screening and Production Optimization of a New Organic Solvent-Tolerance Fungal Lipase
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 194
EP  - 201
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.33
DO  - 10.2991/bbe-16.2016.33
ID  - Peng2016/07
ER  -