Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion

DOI

10.2991/bbe-16.2016.30How to use a DOI?

Keywords

Trf2 gene, cDNA cloning, Real-time fluorescence quantitative PCR, Bioinformation prediction.

Abstract

In order to study Trf 2 protein function in the sperm production, the Trf2 gene was studied and the protein function was predicted in this paper. Based on blast searches with the mRNA sequence of human Trf 2 and the bovine-EST databases, we used fragment assembly and RT-PCR technology to amplify sequence. The results show that a 1701 bp cDNA fragment (GenBank accession No.EU140625) isfirst obtained. The sequence contains a 561 bp ORF encoding 186 amino acids. Homology analysis show that the amino acid sequences of bovine Trf 2 is highly homologous to other species. Real-time F Q- PCR demonstrates that the levelof Trf 2 gene in liver and testis is highest in all organizations, relative to muscle. The protein may be associated with spermatogenesis. We acquire the Trf2 gene sequences and predict the function of Trf2 protein.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yahong Huai
AU  - Shangzhong Xu
PY  - 2016/07
DA  - 2016/07
TI  - Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 177
EP  - 182
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.30
DO  - 10.2991/bbe-16.2016.30
ID  - Huai2016/07
ER  -