Effects of Adventitious Buds Regeneration From Three Kinds of Explants in Jatropha Curcas

DOI

10.2991/bbbs-18.2018.48How to use a DOI?

Keywords

Jatropha curcas, Regeneration, Adventitious Buds, Thidiazuron.

Abstract

An efficient method has been established to induce adventitious buds regeneration from 3 kinds of explants (hypocotyl, cotyledon leaf and leaf) of Jatropha curcas L. All the explants were treated by high concentrations (5??"120 mg/L) of TDZ solution for different soak period (5??"80 min) with the regeneration rate significantly enhanced and the quality of the regenerated buds dramatically improved. The best results of adventitious buds regeneration were detected when explants were treated with 20 mg/L TDZ solution for 20 min before being inoculated on basic MS medium free of hormone for 30 days. What’s more, addition of 0.4 mg/L GA3 into the elongation medium was the most conducive to the extension of adventitious buds. By applying this method described in our paper, regeneration effect of J. curcas was remarkably improved.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jianian Tang
AU  - Guoxuan Liu
AU  - Fuguang Yang
AU  - Chuanteng Huang
AU  - Kaiyuan Zhou
AU  - Sufang Niu
AU  - Shaoxia Yang
AU  - Yali Yang
AU  - Ying Liu
PY  - 2018/04
DA  - 2018/04
TI  - Effects of Adventitious Buds Regeneration From Three Kinds of Explants in Jatropha Curcas
BT  - Proceedings of the 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018)
PB  - Atlantis Press
SP  - 230
EP  - 235
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbbs-18.2018.48
DO  - 10.2991/bbbs-18.2018.48
ID  - Tang2018/04
ER  -