The Growth Curve and Total Colonies Number of Lactobacillus spp on the Pink Guava (Psidium guajava Linnaeus) Juice Medium

DOI

10.2991/ahsr.k.210723.019How to use a DOI?

Keywords

colony number, growth curve, lactobacillus spp, pink guava juice

Abstract

Probiotics are bacteria that are beneficial to human health. Probiotic drinks from cow milk medium use carbohydrates, vitamins, and minerals for its fermentation process. Pink guava fruit has a higher content of fructose, glucose, sucrose, vitamins (B1-B3) and phosphorus and is non-cholesterol than cow milk. This study aims to find out the total number of colonies and an overview of the growth curve of Lactobacillus spp cultured in the medium of pink guava juice (Psidium guajava Linnaeus) in vitro and then compared with the Indonesian national standard for probiotic drinks (107 CFU/ml). The growth of Lactobacillus spp in pink guava medium observations every 12 hours for 60 hours. The method used for calculating the number of colonies is the TPC (Total Plate Count) method then described in the form of a growth curve. The results showed the growth curve Lactobacillus spp in the media of pink guava juice from the log phase until the death phase starting at the 12th hour, 24, 36, 48 to 60 with TPC amounts of 21.5x1010CFU/ml, 49x1010CFU/ml, 49.5x1010CFU/ml, 25.5 x1010CFU/ml and 13x1010 CFU/ml respectively. The stationary phase on the curve begins at the 24th to the 36th hour. The conclusion of this research is that the pink guava juice media can be an excellent growth medium for Lactobacillus spp the conclusion of this research is that the pink guava juice media can be an excellent growth medium for Lactobacillus spp.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Eka Noneng Nawangsih
AU  - Putri Heryani Kusnawan
AU  - Maman Djamaludin
PY  - 2021
DA  - 2021/07/24
TI  - The Growth Curve and Total Colonies Number of Lactobacillus spp on the Pink Guava (Psidium guajava Linnaeus) Juice Medium
BT  - Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)
PB  - Atlantis Press
SP  - 76
EP  - 79
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210723.019
DO  - 10.2991/ahsr.k.210723.019
ID  - Nawangsih2021
ER  -