Development and evaluation of a DNA detection Kit on Identification of Agkistrodon Authenticity Based on Bioinformatics

DOI

10.2991/ameii-15.2015.160How to use a DOI?

Keywords

Agkistrodon; DNA Kit; PCR; bioinformatics

Abstract

We designed and evaluated a kind of Agkistrodon DNA detection kit. Bioinformatics was used to investigate the characteristics of cytochrome b (cyt b ) gene in Agkistrodon. A series of primers were designed, and sequencing and blasting in conjunction with PCR technology were applied. Its’ indexes including specificity, sensitivity and repeatability were evaluated according to the method recorded in Chinese Pharmacopoeia (2010 editions). The kit assay proved effective after repeated freezing and thawing 20 times, and repeatablity test indicated the same data through three times. The lowest amount was 0.025g of each tested specimen. The specificity test confirmed that 14 (77.8%) were real, and 4 was counterfeits in all samples. The developed DNA kit was accurate, effective in identification of Agkistrodon, compared with the Pharmacopoeia method.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Linlin Ma
AU  - Wenjing Yu
AU  - Wei Chu
AU  - Xiaohan Li
AU  - Mingcheng Li
PY  - 2015/04
DA  - 2015/04
TI  - Development and evaluation of a DNA detection Kit on Identification of Agkistrodon Authenticity Based on Bioinformatics
BT  - Proceedings of the International Conference on Advances in Mechanical Engineering and Industrial Informatics
PB  - Atlantis Press
SP  - 861
EP  - 866
SN  - 2352-5401
UR  - https://doi.org/10.2991/ameii-15.2015.160
DO  - 10.2991/ameii-15.2015.160
ID  - Ma2015/04
ER  -