Study for Rapid Determination of Tetrodotoxin Based on Lateral Flow Immunoassay

DOI

10.2991/ame-16.2016.212How to use a DOI?

Keywords

Tetrodotoxin; Lateral flow immunoassay, Superparamagnetic nanoparticle probe, Rapid detection.

Abstract

Trichloroacetic acid (TCA) was used as a protein precipitation in the pretreatment process. The content of TCA in the sample was optimized. Lateral flow immunoassay (LFIA) based on superparamagnetic nanoparticle (SPMNP) probe for TTX detection was employed as a detection method. This article is intended to establish a fast, easy, effective TTX detection method and a matching pretreatment method that could be applied to on-site test. It is found that the obtained results were identical with the reference analytical values after the sample treated by 12% TCA and with 5:1:1 ratio of 10% Tween-20 in BS to 2g/mL BSA in BS to 0.35g/mL sucrose in BS combined. The detection limit of TTX-spiked samples was found to be 6 ng/mL. The whole assay time of the qualitative test for TTX detection was less than 1.5h.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jun Chen
AU  - Jing Zhao
AU  - Ning-Ping Tao
AU  - Jian Cong
PY  - 2016/06
DA  - 2016/06
TI  - Study for Rapid Determination of Tetrodotoxin Based on Lateral Flow Immunoassay
BT  - Proceedings of the 2nd Annual International Conference on Advanced Material Engineering (AME 2016)
PB  - Atlantis Press
SP  - 1305
EP  - 1314
SN  - 2352-5401
UR  - https://doi.org/10.2991/ame-16.2016.212
DO  - 10.2991/ame-16.2016.212
ID  - Chen2016/06
ER  -