Adaptive Measured Model for Cell Adhesive Force Using Dielectrophoresis Force

DOI

10.2991/aiie-15.2015.109How to use a DOI?

Keywords

Polydimethylsiloxane; Endothelial cell; Dielectrophoresis Force; Cell adhesive force

Abstract

The Human Umbilical Vein Endothelial Cell Line (ECV304) was seeded on the polydimethylsiloxane(PDMS). The object of this study is to find out the adaptive measurement of cell adhesive force, which works in the optimal environment parameter, such as solution, working frequency, collagen smearing etc., to avoid the bubble formation in the solution. The result showed the cells seeded on the large area substrate with 2 mm structural spacing and the small area substrate with 100 m structural spacing have better adhesive force. It was also clear to find that large area substrates also showed faster cell growth and expansion. They are more suitable as culture substrates for the measurement of cell adhesion force. As for work solution, 2% glucose solution that has relative low conductivity and concentration has the best measurement that effectively obtained cell adhesion force.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - C.C. Lien
AU  - C. Ay
AU  - J.Y. Cheng
AU  - C.W. Young
PY  - 2015/07
DA  - 2015/07
TI  - Adaptive Measured Model for Cell Adhesive Force Using Dielectrophoresis Force
BT  - Proceedings of the 2015 International Conference on Artificial Intelligence and Industrial Engineering
PB  - Atlantis Press
SP  - 399
EP  - 402
SN  - 1951-6851
UR  - https://doi.org/10.2991/aiie-15.2015.109
DO  - 10.2991/aiie-15.2015.109
ID  - Lien2015/07
ER  -