Construction and Identification of Eukaryotic Expression Vector of Vlgr1

DOI

10.2991/aetr-17.2018.81How to use a DOI?

Keywords

Vlgr1; Vector; Construction; Identification; Expression

Abstract

To construct the Vlgr1 plasmid containing the functional domain, provide experimental material for the study of Vlgr1 - mediated signaling pathways and hydrolysis mechanisms. Methods: Primers were designed according to the gene sequences in the Gene bank, the DNA sequences of different domain regions were cloned and inserted into vector -pEGFP -N1 to construct Vlgr1--pEGFP -N1 expression plasmid, and then transfected into 293T cell line by double digestion, PCR and sequencing. The transfection was detected by fluorescence and Western Blot, the expression of Vlgr1 gene was observed. Results: It was confirmed that the plasmid was successfully expressed by gene sequencing, fluorescence method and Western Blot method. Conclusions: The Vlgr1 plasmid containing the functional domain was successfully constructed.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ping Zeng
AU  - Xiaoming Huang
AU  - Xueli Cheng
AU  - Anping Wang
AU  - Chunling Zhao
AU  - Junhong Dong
PY  - 2018/03
DA  - 2018/03
TI  - Construction and Identification of Eukaryotic Expression Vector of Vlgr1
BT  - Proceedings of the 2017 International Conference Advanced Engineering and Technology Research (AETR 2017)
PB  - Atlantis Press
SP  - 420
EP  - 423
SN  - 2352-5401
UR  - https://doi.org/10.2991/aetr-17.2018.81
DO  - 10.2991/aetr-17.2018.81
ID  - Zeng2018/03
ER  -