Separation and Purification of Three Main Lignans from the Anti-Inflammatory Extract of Lllicium Difengpi by High-Speed Counter-Current Chromatography

DOI

10.2991/aeece-16.2016.9How to use a DOI?

Keywords

Illicium difengpi; Lignans; HSCCC; Magnolol

Abstract

Based on the principle of partition coefficient values ( ) for target compounds and the separation factor ( ) between target compounds, the optimal two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.3:1:0.2, v/v) was applied for the first time to isolate and purify three main lignans from the anti-inflammatory active extract of bark of Illicium difengpi. Using upper phase as the mobile phase in the head to tail elution model, magnolol (297.6 mg), difengpin (15.7 mg), and isodunnianol (17.5 mg), with the purities of more than 95%, were obtained from 800 mg of crude extract in a single operation. Additionally, their structures were identified by MS, 1H-NMR and 13C-NMR.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Desheng Ning
AU  - Yixia Fu
AU  - Zhenghong Pan
PY  - 2016/07
DA  - 2016/07
TI  - Separation and Purification of Three Main Lignans from the Anti-Inflammatory Extract of Lllicium Difengpi by High-Speed Counter-Current Chromatography
BT  - Proceedings of the 2016 2nd International Conference on Advances in Energy, Environment and Chemical Engineering (AEECE 2016)
PB  - Atlantis Press
SP  - 40
EP  - 45
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeece-16.2016.9
DO  - 10.2991/aeece-16.2016.9
ID  - Ning2016/07
ER  -