Extraction and Antioxidant Activity of Flavones from Salvia miltiorrhiza

DOI

10.2991/aeece-15.2015.168How to use a DOI?

Keywords

Salvia miltiorrhiza; flavones; Ultrasonic extraction; antioxidation activity

Abstract

Objective: Salvia miltiorrhiza is a traditional Chinese herb medicine and is widely used in clinic application. This study is intended to extract flavones from Salvia miltiorrhiza by ultrasonic method in order to evaluate its antioxidation activity. 2. Methods: (1) Spectrophotometry is used to determine content of flavones from Salvia miltiorrhiza; (2) Ultrasonic method was used to extract flavones from Salvia miltiorrhiza and 80% ethanol-water as the solution. The effect of the different volume of solvent, extraction time and frequency were conducted (3) The antioxidation of Salvia miltiorrhiza flavones is evaluated by pyrogallol autoxidation and deoxyribose degradation. 3. Results: (1) Phenol - sulfuric acid method was established to test the content of flavones from Salvia miltiorrhiza. The results were in good linear relationship, and y = 0.0119 x - 0.0061, R2 = 0.9996. (2) The extraction process of Salvia miltiorrhiza flavones by ultrasonic method was 5g raw material, 245mL 80% ethanol-water solution, twice extraction, 40 min for each. (3) Antioxidtion tests indicated that with the concentration 0.05mg/mL of flavones, the inhibition rate for superoxide anion radical could reach 76.89% and 58.54% for hydroxyl radical, respectively. And the reducing power was likely to increase with the increasing of flavones concentration. 4. Conclusion: Ultrasonic method could effectively extract flavones from Salvia miltiorrhiza. Antioxidation tests indicate that flavones from Salvia miltiorrhiza have a certain removal effect on superoxide anion radical and hydroxyl radical.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Guangtao Jia
PY  - 2015/09
DA  - 2015/09
TI  - Extraction and Antioxidant Activity of Flavones from Salvia miltiorrhiza
BT  - Proceedings of the International Conference on Advances in Energy, Environment and Chemical Engineering
PB  - Atlantis Press
SP  - 818
EP  - 823
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeece-15.2015.168
DO  - 10.2991/aeece-15.2015.168
ID  - Jia2015/09
ER  -