The Effect of Bengle (Zingiber Cassumunar Roxb.) Rhizome Chloroform Extract on Nitric Oxide and Reactive Oxygen Intermediate Secretions in Vitro

DOI

10.2991/adics-phs-19.2019.9How to use a DOI?

Keywords

bangle, immunomodulatory, NO secretion, ROI secretion

Abstract

The immune system is needed by the body to maintain its integrity against the dangers caused by various substances in the environment. Immunomodulators are substances that can regulate the immune system and increase immunity. Bangle (Zingiber cassumunar Roxb.) Provides an immunomodulatory effect with an increase in phagocytic activity of macrophages and an increase in NO and ROI. This study aims to see whether there is an increase in NO and ROI secretion on macrophage of mice in vitro. Bangle rhizome was macerated using chloroform. Qualitative analysis by TLC method. The experimental study using macrophages isolated from male Balb mice divided into three concentrated variations 25, 50, 100 µg/ml. Quantitative analysis of NO secretion levels was done by Greiss reaction assay and analysis of ROI secretion levels by NBT reduction assay. The results showed that with the administration of bangle chloroform extract concentrations of 25, 50, 100 µg/ml had a significant effect (p<0.05) on increasing NO secretion and ROI compared with the negative control group. In NO there was an increase in NO secretion levels with values in the control group, bangle chloroform extract groups concentrations of 25, 50, and 100 µg / ml respectively 11.8972µM, 17.7265µM, 19.4754µM and 22.3900µM. In ROI there was an increase in percentage ROI secretion with values in the control group, ERB groups concentrations of 25, 50, and 100 µg / ml respectively were 14.67%, 20.67%, 24%, and 27.3%. In conclusion, administration of bangle rhizome extract of with concentrations 25, 50, 100 µg/ml has significant effect (p< 0,05) to increase the percentage of ROI secretion and NO secretion level of male Balb/C mice macrophages in vitro

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Nurkhasanah Nurkhasanah
AU  - Nanik Sulistyani
AU  - Mifta Annisa Ghifarizi
PY  - 2019/11
DA  - 2019/11
TI  - The Effect of Bengle (Zingiber Cassumunar Roxb.) Rhizome Chloroform Extract on Nitric Oxide and Reactive Oxygen Intermediate Secretions in Vitro
BT  - Proceedings of the 2019 Ahmad Dahlan International Conference Series on Pharmacy and Health Science (ADICS-PHS 2019)
PB  - Atlantis Press
SP  - 100
EP  - 104
SN  - 2468-5739
UR  - https://doi.org/10.2991/adics-phs-19.2019.9
DO  - 10.2991/adics-phs-19.2019.9
ID  - Nurkhasanah2019/11
ER  -