Clinical Hematology International

Volume 1, Issue 2, June 2019, Pages 120 - 123

Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance

Authors
Raffaella Greco1, , Maria Chiara Barbanti1, , Nicasio Mancini2, Laura Infurnari2, Renée Pasciuta2, Alessandra Forcina1, Chiara Oltolini3, Gabriele Casirati1, Daniele Mannina1, Fabio Giglio1, Carlo Messina1, Mara Morelli1, Francesca Lorentino1, Sara Mastaglio1, Tommaso Perini1, Luca Vago1, Paolo Scarpellini3, Matteo Giovanni Carrabba1, Maria Teresa Lupo Stanghellini1, Sarah Marktel1, Andrea Assanelli1, Massimo Bernardi1, Consuelo Corti1, Jacopo Peccatori1, Massimo Clementi2, 4, , Fabio Ciceri1, 4, *,
1Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy
2Laboratory of Microbiology and Virology, IRCCS San Raffaele Scientific Institute, Milano, Italy
3Infectious Disease Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy
4Vita-Salute San Raffaele University, Milano, Italy

Equal contribution

*Corresponding author. Email: ciceri.fabio@hsr.it
Corresponding Author
Fabio Ciceri
Received 20 February 2019, Accepted 18 March 2019, Available Online 11 June 2019.
DOI
10.2991/chi.d.190321.001How to use a DOI?
Keywords
Sepsis; Hematological patients; Molecular diagnosis; Carbapenem resistance
Abstract

Infections are a major cause of morbidity and mortality in hematological patients. We prospectively tested a new molecular assay (Verigene®) in 79 consecutive hematological patients, with sepsis by gram-negative bacteria. A total of 82 gram-negative microorganisms were isolated by blood cultures, of which 76 cases were mono-microbial. Considering the bacteria detectable by the system, the concordance with standard blood cultures was 100%. Resistance genes were detected in 20 of the isolates and 100% were concordant with the phenotypic antibiotic resistance. Overall, this new assay correctly identified 66/82 of all the gram-negative pathogens, yielding a general sensitivity of 80.5%, and providing information on genetic antibiotic resistance in a few hours. This new molecular assay could ameliorate patient management, resulting in a more rational use of antibiotics.

Copyright
© 2019 International Academy for Clinical Hematology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal
Clinical Hematology International
Volume-Issue
1 - 2
Pages
120 - 123
Publication Date
2019/06/11
ISSN (Online)
2590-0048
DOI
10.2991/chi.d.190321.001How to use a DOI?
Copyright
© 2019 International Academy for Clinical Hematology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - Raffaella Greco
AU  - Maria Chiara Barbanti
AU  - Nicasio Mancini
AU  - Laura Infurnari
AU  - Renée Pasciuta
AU  - Alessandra Forcina
AU  - Chiara Oltolini
AU  - Gabriele Casirati
AU  - Daniele Mannina
AU  - Fabio Giglio
AU  - Carlo Messina
AU  - Mara Morelli
AU  - Francesca Lorentino
AU  - Sara Mastaglio
AU  - Tommaso Perini
AU  - Luca Vago
AU  - Paolo Scarpellini
AU  - Matteo Giovanni Carrabba
AU  - Maria Teresa Lupo Stanghellini
AU  - Sarah Marktel
AU  - Andrea Assanelli
AU  - Massimo Bernardi
AU  - Consuelo Corti
AU  - Jacopo Peccatori
AU  - Massimo Clementi
AU  - Fabio Ciceri
PY  - 2019
DA  - 2019/06/11
TI  - Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance
JO  - Clinical Hematology International
SP  - 120
EP  - 123
VL  - 1
IS  - 2
SN  - 2590-0048
UR  - https://doi.org/10.2991/chi.d.190321.001
DO  - 10.2991/chi.d.190321.001
ID  - Greco2019
ER  -