P4.31 ARTERIAL STIFFNESS AND HAEMOSTASIS CHANGES IN OBESE ZUCKER RATS

DOI

10.1016/j.artres.2013.10.149How to use a DOI?

Abstract

Objectives: The metabolic syndrome (MetS), a cluster of risk factor including abdominal fat, hypertension, dyslipidemia and raised fasting plasma glucose, is associated with modifications in the arterial wall, endothelial dysfunction and atherothrombosis. To explore the course of events we characterized the coagulation, fibrinolysis and vascular phenotypes in 25 and 80-week-old Zucker rats, that mimics human MetS.

Methods: Arterial phenotype was assessed using ultrasonic echo-tracking. Thrombin generation was monitored using calibrated automated thrombography. Fibrinolysis was measured by a clot lysis assay.

Results: Endothelial dysfunction was evidenced by a high plasma concentration of von Willebrand factor at both ages. The arterial wall stress/modulus curves were superimposed at 25 weeks and shifted towards the left with age, the shift being more pronounced in obese rats. Media thickness was not modified with MetS but was increased with aging in obese and lean rats. In vitro thrombin generation was higher in 25-week-old obese rats than in age-matched control lean rats (428±29 versus 328±27 nM.min) and still higher at 80 weeks (422±30 versus 306±11 nM.min). Regarding fibrinolysis, half-time lysis clot was increased in obese rats compared to control lean rats (46.5±1.2 versus 41.5±0.7 min at 25 weeks) and increased with age (54.1±1.1 versus 49.3±1.8 min at 80 weeks).

Conclusions: We have shown that thrombin generation increased and fibrinolysis decreased in vitro with obesity as early as 25 weeks of age. Theses alterations of hemostasis may participate to the accelerated arterial aging as assessed by increased arterial stiffness triggered by obesity and metabolic disorders in SMet.

Open Access

This is an open access article distributed under the CC BY-NC license.

TY  - JOUR
AU  - J.L. Lagrange
AU  - L.W. Walton
AU  - S.B. Bloemen
AU  - K.C. Cruickshank
AU  - A.B. Benetos
AU  - P.L. Lacolley
AU  - V.R. Regnault
PY  - 2013
DA  - 2013/11/11
TI  - P4.31 ARTERIAL STIFFNESS AND HAEMOSTASIS CHANGES IN OBESE ZUCKER RATS
JO  - Artery Research
SP  - 144
EP  - 144
VL  - 7
IS  - 3-4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2013.10.149
DO  - 10.1016/j.artres.2013.10.149
ID  - Lagrange2013
ER  -