P141 COMPARISON BETWEEN TECHNIQUES OF EVALUATION MICROVASCULAR MORPHOLOGY: THE GOLD-STANDARD LOCALLY INVASIVE MICROMYOGRAPHY VS. THREE NON-INVASIVE TECHNIQUES. PRELIMINARY DATA

DOI

10.1016/j.artres.2017.10.153How to use a DOI?

Abstract

Objective: The gold standard technique of evaluation microvascular morphology in human is generally considered the measure of media to lumen ratio (M/L) of subcutaneous small vessels obtained by local biopsies and evaluated by wire or pressure micromyography. However, non-invasive techniques for the evaluation of retinal arterioles were recently proposed, in particular two approaches seem to provide interesting information: Scanning Laser Doppler Flowmetry (SLDF) and adaptive optics (AO); both of them provide an estimation of the wall to lumen ratio (WLR) of retinal arterioles. A non-invasive measurement of basal and total capillary density may be obtained by videomicroscopy/capillaroscopy. No direct comparison of the non-invasive techniques in the same population was previously performed, in particular AO was never validated against micromyography.

Methods: In the present study, we enrolled 12 normotensive subjects and 8 hypertensive patients undergoing an election surgical intervention; (11/20 were severely obese). All patients underwent a biopsy of subcutaneous fat during surgery. Subcutaneous small resistance artery structure was assessed by wire myography and the M/L was calculated. WLR of retinal arterioles was obtained by SLDF and AO (SLDF, Heidelberg Engineering, Heidelberg, Germany and RTX-1, Imagine Eyes, Orsay, France). Functional (basal) and structural (total) microvascular density were evaluated by capillaroscopy before and after venous congestion.

Results: The results are summarized in the Table (slope of the relation: p < 0.01 RTX-1 vs. SLDF).

	Basal capillary density in the nailfold/M/L	Total capillary density in the forearm / ML	Basal capillary density in the dorsum of the finger / M/L	Total capillary density in the dorsum of the finger /ML
Correlation coefficients (n = 20)	0.53, r2 = 0.28, p < 0.05	0.50, r2 = 0.25, p < 0.05	0.17, r2 = 0.29, p = NS	0.34, r2 = 0.12, p = NS
	W/L retinal arterioles (SLDF)/ M/L	W/L retinal arterioles (RTX- 1)/M/L	W/L retinal arterioles (SLDF)/ W/L retinal arterioles (RTX-1)
Correlation coefficients (n = 20)	0.54, r2 = 0.29, p < 0.01	0.90, r2 = 0.81, p < 0.001	0.71, r2 = 0.50, p < 0.001

Conclusions: Our data suggest that AO has a substantial advantage over SLDF in terms of evaluation of microvascular morphology, since it is more closely correlated with the M/L of subcutaneous small arteries, considered a gold-standard approach.

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TY  - JOUR
AU  - C. De Ciuceis
AU  - S. Caletti
AU  - M.A. Coschignano
AU  - C. Rossini
AU  - S. Duse
AU  - F. Docchio
AU  - S. Pasinetti
AU  - F. Zambonardi
AU  - F. Semeraro
AU  - G. Sansoni
AU  - C. Agabiti Rosei
AU  - P. Pileri
AU  - E. Agabiti Rosei
AU  - D. Rizzoni
PY  - 2017
DA  - 2017/12/06
TI  - P141 COMPARISON BETWEEN TECHNIQUES OF EVALUATION MICROVASCULAR MORPHOLOGY: THE GOLD-STANDARD LOCALLY INVASIVE MICROMYOGRAPHY VS. THREE NON-INVASIVE TECHNIQUES. PRELIMINARY DATA
JO  - Artery Research
SP  - 95
EP  - 96
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.153
DO  - 10.1016/j.artres.2017.10.153
ID  - DeCiuceis2017
ER  -