P78 LONGITUDINAL MICRO-ULTRASOUND ASSESSMENT OF THE OB/OB MOUSE MODEL: EVALUATION OF CARDIOVASCULAR, RENAL AND HEPATIC PARAMETERS

Nicole Di Lascio; Claudia Kusmic; Francesco Stea; Francesca Lenzarini; Cristina Barsanti; Arthur Leloup; Francesco Faita

doi:10.1016/j.artres.2017.10.094

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Volume 20, Issue C, December 2017, Pages 74 - 75

P78 LONGITUDINAL MICRO-ULTRASOUND ASSESSMENT OF THE OB/OB MOUSE MODEL: EVALUATION OF CARDIOVASCULAR, RENAL AND HEPATIC PARAMETERS

Authors

Nicole Di Lascio¹^{, 2}, Claudia Kusmic¹, Francesco Stea³, Francesca Lenzarini¹, Cristina Barsanti¹, Arthur Leloup⁴, Francesco Faita¹

¹Institute of Clinical Physiology, CNR, Pisa, Italy

²Institute of Life Sciences, Scuola Superiore Sant’Anna, Pisa, Italy

³Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

⁴Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium

Available Online 6 December 2017.

DOI: 10.1016/j.artres.2017.10.094 How to use a DOI?
Abstract: Purpose/Background/Objectives: Obesity is associated with increased risk factors for cardiovascular disease (CVD). Leptin-deficient mice (ob/ob) are widely employed to investigate obesity. This study is aimed at providing a micro-ultrasound (mUS) longitudinal evaluation of the ob/ob mouse in terms of cardiovascular effects, consequences for renal microcirculation and liver fat accumulation.

Methods: Sixteen wild-type (wt) and eleven ob/ob male mice were studied at 8 (T₀) and 25 (T₁) weeks of age with a mUS system (Vevo2100). Cardiac output (CO), ejection fraction (EF), stroke volume (SV), fractional shortening (FS) and E/A ratio were measured from cardiac images. Mean diameter (Dm_abd, Dm_car), relative distension (relD_abd, relD_car) and pulse wave velocity (PWV_abd, PWV_car) were obtained for both abdominal aorta and common carotid. Renal resistivity and pulsatility index (RI, PI) were assessed. The ratio between grey-levels related to liver and kidney (Steato-Score) was used as index of hepatic steatosis.

Results: At T₀, ob/ob mice showed reduced SV, EF, CO and relD_abd values and increased LVmass, PWV_abd, RI, PI and Steato-score. Similar results for SV, EF, CO, RI, PI and Steato-Score were found at T₁; furthermore, obese mice showed reduced Dm_abd and Dm_car measurements in comparison with lean controls. The longitudinal analysis showed an increase in LVmass and Dm_abd and a reduction of FS, EF, CO, relD_abd and relD_car for wt animals and no differences for the ob/ob one.

Conclusions: ob/ob mice presented a premature cardiac dysfunction without a further age-related deterioration and a reduction in the abdominal aorta and carotid artery mean diameter in adult age.
Open Access: This is an open access article distributed under the CC BY-NC license.

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Journal: Artery Research
Volume-Issue: 20 - C
Pages: 74 - 75
Publication Date: 2017/12/06
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2017.10.094 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

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ris enw bib

TY  - JOUR
AU  - Nicole Di Lascio
AU  - Claudia Kusmic
AU  - Francesco Stea
AU  - Francesca Lenzarini
AU  - Cristina Barsanti
AU  - Arthur Leloup
AU  - Francesco Faita
PY  - 2017
DA  - 2017/12/06
TI  - P78 LONGITUDINAL MICRO-ULTRASOUND ASSESSMENT OF THE OB/OB MOUSE MODEL: EVALUATION OF CARDIOVASCULAR, RENAL AND HEPATIC PARAMETERS
JO  - Artery Research
SP  - 74
EP  - 75
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.094
DO  - 10.1016/j.artres.2017.10.094
ID  - DiLascio2017
ER  -

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