Artery Research

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Volume 20, Issue C, December 2017, Pages 63 - 63

P5 ERYTHROCYTE DEFORMABILITY AND NITRIC OXIDE PRODUCTION IN ANIMAL MODEL OF PRIMARY HYPERTENSION AND THEIR AGE-DEPENDENT CHANGES

Authors
Jana Radosinska1, 2, Peter Balis3, Angelika Puzserova3
1Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Slovak Republic
2Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic
3Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovak Republic
Available Online 6 December 2017.
DOI
10.1016/j.artres.2017.10.058How to use a DOI?
Abstract

Objectives: Reduced deformability of red blood cells (RBC) plays an important role in etiology of various diseases including cardiovascular. The nitric oxide (NO) was identified as one of factors responsible for maintenance of RBC deformability. Reduced bioavailability of NO might be important in the pathogenesis of hypertension. The aim of present study was to determine the effect of hypertension and aging on RBC deformability and NO production of experimental animals.

Methods: Spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were divided into 6 groups according to age (7, 20 and 52 weeks) and strain: SHR-7, SHR-20, SHR-52 and WKY-7, WKY-20, WKY-52. Blood was used for determination of RBC deformability using filtration method and NO production in RBCs using fluorescent NO probe DAF-2 DA.

Results: We found reduced deformability at WKY-52 and SHR-52 as compared to strain- matched 20-week-old animals. Strain-related differences in deformability were observed at 7 and 52 weeks of age, where the SHR-7 had reduced deformability and the SHR-52 had increased deformability as compared to age-matched WKY. We have found that at younger age, deformability and NO production in RBCs was able to increase, while in the older age there was a decrease in both parameters.

Conclusions: Changes in the RBC deformability under hypertensive conditions are unlikely to be related to changes in NO production. On the other hand, age-related changes in deformability of both, WKY and SHR are at least partially associated with changes in NO production. Supported by grants VEGA 1/0032/14 and Slovak Society of Cardiology.

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Journal
Artery Research
Volume-Issue
20 - C
Pages
63 - 63
Publication Date
2017/12/06
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2017.10.058How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

risenwbib
TY  - JOUR
AU  - Jana Radosinska
AU  - Peter Balis
AU  - Angelika Puzserova
PY  - 2017
DA  - 2017/12/06
TI  - P5 ERYTHROCYTE DEFORMABILITY AND NITRIC OXIDE PRODUCTION IN ANIMAL MODEL OF PRIMARY HYPERTENSION AND THEIR AGE-DEPENDENT CHANGES
JO  - Artery Research
SP  - 63
EP  - 63
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.058
DO  - 10.1016/j.artres.2017.10.058
ID  - Radosinska2017
ER  -