4.2 DISCREPANCY BETWEEN IN-VIVO MEASURE AND EX-VIVO CALCULATION OF PULSE WAVE VELOCITY IN RETINAL ARTERIES

Mahdieh Rezaeian; Arthur Leloup; Angela Schulz; Mojtaba Golzan; Stuart Graham; Alberto P. Avolio; Mark Butlin

doi:10.1016/j.artres.2018.10.041

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Volume 24, Issue C, December 2018, Pages 75 - 75

4.2 DISCREPANCY BETWEEN IN-VIVO MEASURE AND EX-VIVO CALCULATION OF PULSE WAVE VELOCITY IN RETINAL ARTERIES

Authors

Mahdieh Rezaeian¹, Arthur Leloup², Angela Schulz¹, Mojtaba Golzan³, Stuart Graham¹, Alberto P. Avolio⁴, Mark Butlin⁴

¹Department of Clinical Medicine, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia

²Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium

³Vision Science Group, Graduate School of Health, University of Technology Sydney, Sydney, Australia

⁴Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia

Available Online 4 December 2018.

DOI: 10.1016/j.artres.2018.10.041 How to use a DOI?
Abstract: Background: Pulse wave velocity (PWV) in large arteries is a pressure-dependent marker of arterial stiffness. The retinal vasculature provides unique access to the microcirculation. There is inconsistency between reported values of retinal PWV (rPWV). The pressure dependency of rPWV was measured in-vivo and calculated ex-vivo using retinal artery material properties to investigate the inconsistencies.

Methods: High-speed fundus videos (125 fps) from three Sprague Dawley rats were recorded simultaneously with electrocardiogram and blood pressure. rPWV was measured using the cardiac component of retinal artery diameter waveforms at two retinal sites across a physiological range (70–130 mmHg) of mean arterial pressure (MAP). Ex-vivo tensile testing was performed on bovine retinal arteries, rat retinal arteries being too small for myography. Diameter and wall thickness of the retinal artery adjacent to the optic disc were measured using optical coherence tomography. Tensile testing was performed using a wire myograph in 9 bovine retinal artery specimens.

Results: In-vivo results showed a significant positive correlation between rPWV (4.9 ± 1.8 mm/s) and MAP (R2 = 0.58, p < 0.001) as expected. Ex-vivo, calculated rPWV using material stiffness and geometry measurement ranged between 4.6 and 7.0 m/s at effective distending pressures between 70 and 100 mmHg.

Conclusions: Ex-vivo and in-vivo results differed by three orders of magnitude but should be the same. Ex-vivo results are in the same order as measured in-vivo in large arteries. In-vivo rPWV was lower than expected yet was responsive to changes in MAP. Further studies are required to uncover what rPWV is a measurement of, if not arterial stiffness.
Open Access: This is an open access article distributed under the CC BY-NC license.

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Journal: Artery Research
Volume-Issue: 24 - C
Pages: 75 - 75
Publication Date: 2018/12/04
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2018.10.041 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

Cite this article

ris enw bib

TY  - JOUR
AU  - Mahdieh Rezaeian
AU  - Arthur Leloup
AU  - Angela Schulz
AU  - Mojtaba Golzan
AU  - Stuart Graham
AU  - Alberto P. Avolio
AU  - Mark Butlin
PY  - 2018
DA  - 2018/12/04
TI  - 4.2 DISCREPANCY BETWEEN IN-VIVO MEASURE AND EX-VIVO CALCULATION OF PULSE WAVE VELOCITY IN RETINAL ARTERIES
JO  - Artery Research
SP  - 75
EP  - 75
VL  - 24
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2018.10.041
DO  - 10.1016/j.artres.2018.10.041
ID  - Rezaeian2018
ER  -

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