P8.02 CHARACTERIZATION OF THE VASORELAXANT MECHANISMS OF ENDOCANNABINOID OLEOYLETHANOLAMIDE IN THE BOVINE OPHTHALMIC ARTERY
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- 10.1016/j.artres.2011.10.125How to use a DOI?
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Background: Numerous studies show the potential therapeutic effect of different endocannabinoids and in particular the vasorelaxant effects in several vascular beds
Aim: To evaluate the vasorelaxant effect of oleoylethanolamide on isolated bovine ophthalmic arteries and to evaluate the possible mechanisms involved in relaxant responses.
Methods: Ophthalmic arteries were isolated from bovine eyes and mounted in a wire miograph for isometric tension recording. The effects time- and concentration-dependent were assayed by addition of the oleoylethanolamide to the organ bath.
Results: Oleoylethanolamide (0.1 – 10 μM) produced a significant concentration- and time-dependent vasorelaxation in the bovine ophthalmic artery pre-contracted with 5-HT (1 μM). The removal of endothelium provoked a slight reduction of the relaxant effects. Interestingly, a pre-treatment with antagonist PPARα GW6471 (1 μM) inhibited the concentration- and time-dependent oleoylethanolamide-induced vasorelaxation.
Conclusion: The present study shows that oleoylethanolamide relaxed the isolated bovine ophthalmic artery in the concentration- and time-dependent manner. The candidate responsible of the vasorelaxant response to oleoylethanolamide appear to be the PPARα. This relaxant effect is an exciting tool to prevent ischemic injury because it improves the blood supply to the retina.
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TY - JOUR AU - M.R. Romano AU - A.D. Lograno AU - M.D. Lograno PY - 2011 DA - 2011/11/29 TI - P8.02 CHARACTERIZATION OF THE VASORELAXANT MECHANISMS OF ENDOCANNABINOID OLEOYLETHANOLAMIDE IN THE BOVINE OPHTHALMIC ARTERY JO - Artery Research SP - 183 EP - 183 VL - 5 IS - 4 SN - 1876-4401 UR - https://doi.org/10.1016/j.artres.2011.10.125 DO - 10.1016/j.artres.2011.10.125 ID - Romano2011 ER -