Artery Research

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Volume 5, Issue 4, December 2011, Pages 154 - 154

P2.05 ARE RETINAL MICROVASCULAR PHENOTYPES ASSOCIATED WITH 1675G/A POLYMORPHISM IN ANGIOTENSIN II RECEPTOR-2 GENE?

Authors
Y. Liu1, T. Kuznetsova1, L. Thijs1, Y. Jin1, B. Schmitz2, S.M. Brand2, E. Brand3, P. Manunta4, G. Bianchi4, H. Struijker-Boudier5, J.A. Staessen1, 6
1Studies Coordinating Centre, Labotary of Hypertesion, University of Leuven, Leuven, Belgium
2Medical Faculty of Münster, Department of Molecular Genetics of Cardiovascular Disease, University of Münster, Münster, Germany
3Internal Medicine D, Department of Nephrology, Hypertension and Rheumatology, University Hospital Münster, Münster, Germany
4Division of Nephrology and Dialysis, San Raffaele Scientific Institute, Università Vita-Salute, San Raffaele Hospital, Milan, Italy
5Department of Pharmacology, Maastricht University, Maastricht, Netherlands
6Department of Epidemiology, Maastricht University, Maastricht, Netherlands
Available Online 29 November 2011.
DOI
10.1016/j.artres.2011.10.026How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Background: The X-linked AT2R G1675A polymorphism is located in the short intron 1 of the AT2R gene within a sequence motif conforming to a splice branch site. AT2R is expressed in the human retina, but no previous study examined the association between retinal microvascular phenotypes and the AT2R G1675A polymorphism.

Methods: In 340 subjects randomly selected from a Flemish population (mean age, 51.9 years; 51.5% women), we post-processed retinal images (Canon Cr-DGi) using IVAN software to generate the retinal arteriole and venule equivalents (CRAE and CRVE) and the arteriole-to-venule-ratio (AVR). DNA fragments including the AT2R G1675A, AT1R A1166C, ACE I/D and CYP11B2 C–344T polymorphisms, were amplified by PCR. We applied a mixed model to assess phenotype-genotype associations while accounting for relatedness and covariables.

Results: CRAE, CRVE and AVR averaged 151.9 μm, 215.2 μm and 0.710, respectively. CRAE was 5.5 μm greater in women than men and decreased with age (P<0.05). In multivariable-adjusted analyses, CRAE was higher in hemizygous and homozygous carriers of the AT2R A allele than in their G allele counterparts in both sexes combined (+4.49 μm; P=0.014) and in men (+4.91 μm; P=0.032) with a similar trend in women (+3.41 μm; P=0.14). AVR was increased in the presence of the AT2R A allele compared with AT2R G hemizygotes and homozygotes (+0.024; P=0.0082). The associations of CRAE and CRVE with other polymorphisms was not significant.

Conclusions: Pending confirmation in experimental and epidemiological studies, our findings suggest that diameter of the retinal arterioles might be associated with the AT2R 1675G/A polymorphism.

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Journal
Artery Research
Volume-Issue
5 - 4
Pages
154 - 154
Publication Date
2011/11/29
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2011.10.026How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

risenwbib
TY  - JOUR
AU  - Y. Liu
AU  - T. Kuznetsova
AU  - L. Thijs
AU  - Y. Jin
AU  - B. Schmitz
AU  - S.M. Brand
AU  - E. Brand
AU  - P. Manunta
AU  - G. Bianchi
AU  - H. Struijker-Boudier
AU  - J.A. Staessen
PY  - 2011
DA  - 2011/11/29
TI  - P2.05 ARE RETINAL MICROVASCULAR PHENOTYPES ASSOCIATED WITH 1675G/A POLYMORPHISM IN ANGIOTENSIN II RECEPTOR-2 GENE?
JO  - Artery Research
SP  - 154
EP  - 154
VL  - 5
IS  - 4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2011.10.026
DO  - 10.1016/j.artres.2011.10.026
ID  - Liu2011
ER  -