Gender and endothelial progenitor cell number in middle-aged adults

Brian L. Stauffer; Owen J. MacEneaney; Erich J. Kushner; Jennifer N. Cech; Jared J. Greiner; Christian M. Westby; Christopher A. DeSouza

doi:10.1016/j.artres.2008.10.001

<Previous Article In Issue

Volume 2, Issue 4, November 2008, Pages 156 - 160

Gender and endothelial progenitor cell number in middle-aged adults

Authors

Brian L. Stauffer^a^{, b}^{, c}^{, *}, Owen J. MacEneaney^a, Erich J. Kushner^a, Jennifer N. Cech^a, Jared J. Greiner^a, Christian M. Westby^a, Christopher A. DeSouza^a^{, b}

^aIntegrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado, Boulder, CO, USA

^bDepartment of Medicine, University of Colorado at Denver and the Health Sciences Center, Aurora, CO, USA

^cDepartment of Medicine, Division of Cardiology, Denver Health and Hospital Authority, Denver, CO, USA

^*Corresponding author. Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado at Boulder, UCB 436, Boulder, CO 80309, USA. Tel.: +1 303 724 5440; fax: +1 303 724 5450. E-mail address: brian.stauffer@ucdenver.edu (B.L. Stauffer).

Corresponding Author

Brian L. Stauffer

Received 13 August 2008, Revised 8 October 2008, Accepted 9 October 2008, Available Online 13 November 2008.

DOI: 10.1016/j.artres.2008.10.001 How to use a DOI?
Keywords: Endothelial progenitor cells; Gender; Endothelium
Abstract: Background: Between the ages of 45 and 65 years, the prevalence of cardiovascular disease is significantly lower in women compared with men. Circulating bone marrow-derived endothelial progenitor cells (EPCs) play an important role in vascular repair. Reduced EPC number is predictive of more cardiovascular events. It is currently unknown whether there is a sex-difference in EPC number in middle-aged adults.

Objective: We tested the hypothesis that circulating EPC number is higher in middle-aged women than men.

Methods: Peripheral blood samples were collected from 58 sedentary adults, 29 men (57 ± 1 years) and 29 women (58 ± 1 years). Mononuclear cells were isolated and fluorescence-activated cell sorting (FACS) analysis of cells negative for CD45 was performed for those positive for CD34, and triple positive for CD34, VEGFR-2, and CD133 according to the recommendations of the International Society for Hematotherapy and Graft Engineering.

Results: The number of CD45⁻/CD34⁺ and CD45⁻/CD34⁺/VEGFR-2⁺/CD133⁺ was not significantly different between women and men (0.055 ± 0.006% vs 0.069 ± 0.008% and 0.0013 ± 0.0003% vs 0.0018 ± 0.0004%, respectively).

Conclusions: These results demonstrate no sex-difference in EPC number in middle-age adults. Therefore, it is unlikely that differences in EPC number contribute to the gender-related differences in the prevalence of cardiovascular events in this population.
Open Access: This is an open access article distributed under the CC BY-NC license.

Download article (PDF)
View full text (HTML)

<Previous Article In Issue

Journal: Artery Research
Volume-Issue: 2 - 4
Pages: 156 - 160
Publication Date: 2008/11/13
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2008.10.001 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

Cite this article

ris enw bib

TY  - JOUR
AU  - Brian L. Stauffer
AU  - Owen J. MacEneaney
AU  - Erich J. Kushner
AU  - Jennifer N. Cech
AU  - Jared J. Greiner
AU  - Christian M. Westby
AU  - Christopher A. DeSouza
PY  - 2008
DA  - 2008/11/13
TI  - Gender and endothelial progenitor cell number in middle-aged adults
JO  - Artery Research
SP  - 156
EP  - 160
VL  - 2
IS  - 4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2008.10.001
DO  - 10.1016/j.artres.2008.10.001
ID  - Stauffer2008
ER  -

download .riscopy to clipboard