Artery Research

<Previous Article In Issue
Next Article In Issue>
Volume 24, Issue C, December 2018, Pages 81 - 81

P5 REGIONAL VARIATIONS IN THE MICROMECHANICAL AND BIOCHEMICAL PROPERTIES OF THE OVINE AORTA

Authors
Phakakorn Panpho1, 2, Riaz Akhtar3, Jill Madine4, Mark Field5
1School of Engineering, University of Liverpool, UK
2Faculty of Science and technology, Pibulsongkram Rajabhat University, Phitsanuloke, Thailand
3School of Engineering, University of Liverpool, UK
4Institute of Integrative Biology, University of Liverpool, UK
5Department of Cardiac Surgery, Liverpool Heart and Chest Hospital, UK
Available Online 4 December 2018.
DOI
10.1016/j.artres.2018.10.058How to use a DOI?
Abstract

Background: It is important to understand regional variations in the mechanical and biochemical properties of the aorta to better predict and treat diseases. Although previous studies have been explored regional differences in the structure and biomechanical properties of the aorta, little is known about how these properties vary across its entire length [1, 2].

Objectives: To map the micromechanical and biochemical properties of the ovine aorta from the aortic root to the celiac artery region.

Methods: Fresh ovine aortas (n = 3) were split into nine sections, separated by 2 cm intervals between the aortic root and the celiac artery region. For each section, three biopsies were cut out using a 5 mm biopsy punch (a total of 81 biopsies). An oscillatory nanoindentation method was used to determine the micromechanical properties of the tissue [3]. 16 indents were made per biopsy. The shear storage (G’), the shear loss modulus (G’’) were determined [3]. Subsequently, the same samples were used to determine elastin, collagen and glycosaminoglycan (GAG) levels using established biochemical assays.

Results: Overall, there was a significant correlation between an increase in G’ and collagen (P = 0.01) with distance from the aortic root whilst elastin (P = 0.05) and GAG (P = 0.05) levels were significantly decreased.

Conclusions: Our study is the first to comprehensively map the mechanical and biochemical properties across the entire aorta. There was a progressive increase in mechanical properties from the proximal to the distal region, along with an increase in collagen and a decrease in elastin content.

Open Access
This is an open access article distributed under the CC BY-NC license.

Download article (PDF)
View full text (HTML)

<Previous Article In Issue
Next Article In Issue>
Journal
Artery Research
Volume-Issue
24 - C
Pages
81 - 81
Publication Date
2018/12/04
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2018.10.058How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

risenwbib
TY  - JOUR
AU  - Phakakorn Panpho
AU  - Riaz Akhtar
AU  - Jill Madine
AU  - Mark Field
PY  - 2018
DA  - 2018/12/04
TI  - P5 REGIONAL VARIATIONS IN THE MICROMECHANICAL AND BIOCHEMICAL PROPERTIES OF THE OVINE AORTA
JO  - Artery Research
SP  - 81
EP  - 81
VL  - 24
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2018.10.058
DO  - 10.1016/j.artres.2018.10.058
ID  - Panpho2018
ER  -