Genetic Characterization of Second-Line Drug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis from the Northern Region of India

Rakesh Yadav; Aastha Saini; Jureka Mankotia; Rajiv Khaneja; Priyanka Agarwal; Sunil Sethi

doi:10.2991/j.jegh.2018.02.100

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Volume 8, Issue 3-4, December 2018, Pages 220 - 224

Genetic Characterization of Second-Line Drug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis from the Northern Region of India

Authors

Rakesh Yadav¹, Aastha Saini¹, Jureka Mankotia², Rajiv Khaneja³, Priyanka Agarwal⁴, Sunil Sethi¹^{, *}

¹Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

²Intermediate Reference Laboratory, Dharampur, Himachal Pradesh, India

³State TB Cell, RNTCP, Sector 34, Chandigarh, India

⁴WHO Country Office for India, New Delhi, India

^* Corresponding author. Email: sunilsethi10@hotmail.com

Corresponding Author

Sunil Sethi

Received 14 December 2017, Accepted 26 February 2018, Available Online 31 December 2018.

DOI: 10.2991/j.jegh.2018.02.100 How to use a DOI?
Keywords: Tuberculosis; cross resistance; mutation; MAS PCR; DNA sequencing
Abstract: Rapid detection of drug resistance in Mycobacterium tuberculosis is important for the successful treatment of tuberculosis. Fluoroquinolone and aminoglycoside resistance detection by molecular methods becomes more complex due to cross resistance among them. Thus, we aimed to determine cross-resistance and mutations in resistance genes for these drugs. A total of 336 multidrug-resistant tuberculosis (MDR-TB) cases received in Mycobacteriology laboratory were screened for phenotypic drug sensitivity testing for second-line drugs, i.e., ofloxacin, amikacin, kanamycin, and capreomycin. Molecular characterization of resistance was done by DNA sequencing of gyrA gene for fluoroquinolones (FQ), and multiplex allele-specific polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism (RFLP) of rrs gene for aminoglycosides. Of 336 MDR-TB isolates, 12 were extensively drug-resistant tuberculosis and 219 were sensitive to all the drugs tested. Ofloxacin, amikacin, kanamycin, and capreomycin resistance was detected in 101 (30.1%), 23 (6.8%), 27 (8.1%), and 19 (5.6%) cases, respectively. Eight different mutations were detected in gyrA gene in ofloxacin-resistant isolates and A1401G nucleotide change in rrs gene were seen in 55.6% (15/27), 65.2% (15/23), and 68.4% (13/29) for kanamycin-, amikacin-, and capreomycin-resistant isolates, respectively. Information on second-line drug resistance-associated mutations could potentially be used for development of newer rapid diagnostic tests.
Open Access: This is an open access article under the CC BY-NC license (http://creativecommons.org/licences/by-nc/4.0/).

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Journal: Journal of Epidemiology and Global Health
Volume-Issue: 8 - 3-4
Pages: 220 - 224
Publication Date: 2018/12/31
ISSN (Online): 2210-6014
ISSN (Print): 2210-6006
DOI: 10.2991/j.jegh.2018.02.100 How to use a DOI?
Open Access: This is an open access article under the CC BY-NC license (http://creativecommons.org/licences/by-nc/4.0/).

Cite this article

ris enw bib

TY  - JOUR
AU  - Rakesh Yadav
AU  - Aastha Saini
AU  - Jureka Mankotia
AU  - Rajiv Khaneja
AU  - Priyanka Agarwal
AU  - Sunil Sethi
PY  - 2018
DA  - 2018/12/31
TI  - Genetic Characterization of Second-Line Drug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis from the Northern Region of India
JO  - Journal of Epidemiology and Global Health
SP  - 220
EP  - 224
VL  - 8
IS  - 3-4
SN  - 2210-6014
UR  - https://doi.org/10.2991/j.jegh.2018.02.100
DO  - 10.2991/j.jegh.2018.02.100
ID  - Yadav2018
ER  -

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