CO2 Mitochondrial Gene Identification of Nisaetus cirrhatus as a Part of Indonesian Elang Brontok Genetic Conservation
- DOI
- 10.2991/absr.k.220406.047How to use a DOI?
- Keywords
- Genetic conservation; Indonesia; MT-COI; MT-CO2; Nisaetus cirrhatus
- Abstract
The Elang Brontok, scientifically named as Nisaetus cirrhatus is included in the least Concern in appendix II. The use of the COI gene for taxonomic in our previous study put our samples in a complex species taxonomical position. Further research needs to be done using other mitochondrial genes such as CO2. In addition, the CO2 sequence of Nisaetus cirrhatus has not yet been reported. The purpose of this study was to obtain complete sequences of COI and CO2 and determine the taxonomic position of the Elang Brontok (Nisaetus cirrhatus) in Indonesia. In this study, we used the same three samples as of the previous study for CO1. We designed a pair of primers based on the previously found CO1 sequence with not yet known the length of the target gene since there are no reports for it. Phylogenetic tree reconstruction proved that the Elang Brontok we studied belongs to Nisaetus cirrhatus. The result of combining sequences obtained from this study with one previously reported showed some mutations at the 5’ end of COI. We suggest that the extended 3’ end of COI is part of the yet reported Nisaetus cirrhatus CO2 sequence.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Reni Krisdayana AU - Rizqi Layli Khusufi AU - Dwita Novitasari AU - Annasa Sabatia AU - Delia Wahyu Pangesti AU - Salsabila Kasta Hygiea Iswara AU - Dwi Listyorini PY - 2022 DA - 2022/05/02 TI - CO2 Mitochondrial Gene Identification of Nisaetus cirrhatus as a Part of Indonesian Elang Brontok Genetic Conservation BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 331 EP - 337 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.047 DO - 10.2991/absr.k.220406.047 ID - Krisdayana2022 ER -