Proceedings of the 4th Forum in Research, Science, and Technology (FIRST-T1-T2-2020)

Production of Biodiesel From Unrefined Plants-Derived Oil by Aspergillus oryzae Expressing Bacillus thermocatenulatus Lipase as a Whole-Cell Biocatalyst

Authors
Nova Rachmadona, Martha Aznury, Chiaki Ogino
Corresponding Author
Nova Rachmadona
Available Online 9 February 2021.
DOI
10.2991/ahe.k.210205.060How to use a DOI?
Keywords
biodiesel, Bacillus thermocatenulatus lipase, crude palm oil, enzymatic transesterification
Abstract

In this study, the enzymatic process reaction using a whole-cell biocatalyst was done for biodiesel production of crude plant oils. This work investigated a streamlined technique to convert crude palm oil that consisted high amount of phosphocholine gums. This reaction by using lipase as a catalyst for biodiesel-fuel has an excellent potential compared with chemical reactions. We explored Aspergillus oryzae expressing Bacillus thermocatenulatus lipase (BTL) as a biocatalyst in producing fatty acid methyl FAME (95.54%) obtained at 30 oC, with 5.00 % (w/w) of BTL at 96 h using four molar equivalents of methanol. Therefore, BTL could be useful as whole-cell biocatalyst that can produce biodiesel from feedstock with a high proportion of impurities in industrial application.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 4th Forum in Research, Science, and Technology (FIRST-T1-T2-2020)
Series
Atlantis Highlights in Engineering
Publication Date
9 February 2021
ISBN
10.2991/ahe.k.210205.060
ISSN
2589-4943
DOI
10.2991/ahe.k.210205.060How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Nova Rachmadona
AU  - Martha Aznury
AU  - Chiaki Ogino
PY  - 2021
DA  - 2021/02/09
TI  - Production of Biodiesel From Unrefined Plants-Derived Oil by Aspergillus oryzae Expressing Bacillus thermocatenulatus Lipase as a Whole-Cell Biocatalyst
BT  - Proceedings of the 4th Forum in Research, Science, and Technology (FIRST-T1-T2-2020)
PB  - Atlantis Press
SP  - 358
EP  - 360
SN  - 2589-4943
UR  - https://doi.org/10.2991/ahe.k.210205.060
DO  - 10.2991/ahe.k.210205.060
ID  - Rachmadona2021
ER  -